MS751 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
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€550.00*

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Product number: 305115

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	MS751 is a tumorigenic human cervical carcinoma cell line isolated from the uterus of a female patient with epidermoid carcinoma. The cells were originally obtained from a metastatic lymph node, and they form poorly differentiated epidermoid carcinoma (grade III) when xenografted into nude mice. The tumorigenic and metastatic nature of MS751 cells makes them a valuable model for studying the processes involved in cervical cancer metastasis and tumor progression. These cells are particularly useful for investigating epithelial-to-mesenchymal transition (EMT), invasion, and metastasis, especially in relation to poorly differentiated carcinoma. One of the key molecular features of MS751 is the presence of human papillomavirus (HPV) sequences. Originally reported to contain HPV-18, more recent studies have demonstrated that MS751 cells contain partial sequences of HPV-45, particularly from the E6/E7 region, which are expressed as poly(A)+ RNA. The E6 and E7 oncoproteins are well-known for their roles in disrupting the tumor suppressor functions of p53 and Rb, respectively, which promote uncontrolled cell division and contribute to oncogenesis. The presence of these viral sequences makes MS751 highly relevant for studies on HPV-associated cervical cancers, and specifically for investigating how HPV-45 contributes to the malignancy of cervical cells. MS751 cells exhibit epithelial morphology, which is characteristic of many cervical cancer cell lines. They are widely used for research into the molecular mechanisms underlying HPV-mediated carcinogenesis, as well as for drug discovery and therapeutic screening. Given their metastatic origin and the presence of HPV sequences, MS751 provides an essential model for studying the progression of cervical cancer and testing therapeutic strategies aimed at targeting both viral and tumor-related pathways.
Organism	Human
Tissue	Cervix
Disease	Human papillomavirus-related cervical squamous cell carcinoma
Metastatic site	Lymph node
Synonyms	MS-751, MS 751

Age	47 years
Gender	European
Morphology	Epithelial
Growth properties	Adherent

Citation	MS751 (Cytion catalog number 305115)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_4996

Antigen expression	Blood Type AB, Rh?
Tumorigenic	Yes, in nude mice, forms poorly differentiated epidermoid carcinoma (grade?).
Viruses	HPV18, HPV45

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305115-260525	Certificate of Analysis	21. Jul. 2025	305115
305115-270323	Certificate of Analysis	21. Jul. 2025	305115

MS751 Cells

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Certificate of Analysis (CoA)